THE SYNTHESIS OF NUCLEIC ACID BY VIRUS-INFECTED BACTERIA

The nucleic acid of African Horse-Sickness Virus

Nucleic Acid Economy in Bacteria Infected with Bacteriophage T2

1. The phosphorus content per infective particle of isolated bacteriophage T2 has been redetermined. It does not exceed 1.8 to 2.2 x 10(-11) microg. The equivalent amount of DNA has been defined in terms of several analytical methods and taken as a unit of measurement of intrabacterial DNA. 2. The DNA of E. coli contains guanine, adenine, cytosine, and thymine in approximately equal amounts, bu...

Nucleic acid metabolism of virus-infected HeLa cells.

The study of the chemistry of virus-infected cells cannot yet claim a molecular interpretation for the genesis of the viral cytopathic effect. However, many striking observations have been made that must be accounted for in any molecular theory of viral cytopathology. From the viewpoint of comparative virology, the most consistent viral-induced aberrations involve the nucleic acids. The finding...

Nucleic acid synthesis in cytoplasm of Yaba monkey tumor virus-infected cells.

Yaba tumor virus progeny appeared in cynomolgus monkey kidney cells at 24 h postinfection and reached a plateau at 72 h in the first cycle of replication. Viral DNA synthesis was first detected at about 3 h and reached a peak in 18 h. Maximum coating of viral DNA in infected cells occurred at 4 days postinfection. Rapidly labeled RNA was synthesized in the cytoplasm of virus-infected cells. At ...

The inhibition of nucleic acid synthesis by mycophenolic acid.

1. Mycophenolic acid, an antibiotic of some antiquity that more recently has been found to have marked activity against a range of tumours in mice and rats, strongly inhibits DNA synthesis in the L strain of fibroblasts in vitro. 2. The extent of the inhibition of DNA synthesis is markedly increased by preincubation of the cells with mycophenolic acid before the addition of [(14)C]thymidine. 3....